Version: 1.0.0 | Published: 15 Oct 2026 | Updated: 0 days ago
Documentation
Description:
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From the same subject, fresh dermal and frozen dural cell lines were cultured into a cell culture flask, trypsinized, washed with DPBS−/−, and shipped to GENEWIZ, Inc. (South Plainfield, NJ, USA) for WGS. Bulk DNA was isolated by Qiagen QIAmp DNA Kit and HT DNA Kit. DNA was quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and the DNA integrity was ver- ified with ~1% agarose gel. Libraries were prepped with NEBNext Ultra DNA Library Prep Kit and paired-end 2x150-base-pair sequencing was performed with the Illu- mina HiSeq (Illumina, Inc., San Diego, CA, USA). Reads for each sample were aligned to the reference genome for Homo sapiens (NCBI GRCh38 with decoys) with the Issac Aligner. Mutect2 and Strelka2 were used to call single-nucleotide variants (SNVs) and indels comparing the dermis and dura mater-derived cell lines to each other. Only consensus calls with a PASS value made by both callers were used. The variant allele fraction (VAF) distribution for each cell line was determined through the VCF files generated by Mutect2.
From the same subject, fresh dermal and frozen dural cell lines were cultured into a cell culture flask, trypsinized, washed with DPBS−/−, and shipped to GENEWIZ, Inc. (South Plainfield, NJ, USA) for WGS. Bulk DNA was isolated by Qiagen QIAmp DNA Kit and HT DNA Kit. DNA was quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and the DNA integrity was ver- ified with ~1% agarose gel. Libraries were prepped with NEBNext Ultra DNA Library Prep Kit and paired-end 2x150-base-pair sequencing was performed with the Illu- mina HiSeq (Illumina, Inc., San Diego, CA, USA). Reads for each sample were aligned to the reference genome for Homo sapiens (NCBI GRCh38 with decoys) with the Issac Aligner. Mutect2 and Strelka2 were used to call single-nucleotide variants (SNVs) and indels comparing the dermis and dura mater-derived cell lines to each other. Only consensus calls with a PASS value made by both callers were used. The variant allele fraction (VAF) distribution for each cell line was determined through the VCF files generated by Mutect2.
Coverage
Spatial:
US
Follow Up:
Unknown
Provenance
Temporal
Accrual Periodicity:
Static
Start Date:
01 January 2026
Time Lag:
Not applicable
Accessibility
Access
Access Rights:
-. Access data at:
Usage
Resource Creators:
Andrea R Argouarch, Aimee Kao
Observations
Statistical Population
Population Description
Population Size
Measured Property
Observation Date
Persons
1
Count
01 January 2026