Version: 1.0.0 | Published: 24 Sep 2026 | Updated: 0 days ago
Documentation
Description:
This study deeply characterized a widely available induced pluripotent stem cell (iPSC) lines to rationally select a line that performs well in multiple experimental approaches. We performed an analysis of transcriptional patterns in the pluripotent state, whole genome sequencing, genomic stability after highly efficient CRISPR-mediated gene editing, integrity of the p53 pathway, and differentiation efficiency towards multiple lineages and identified KOLF2.1J as a well-performing cell line. Here, we provide the whole genome and single cell RNA sequencing files from our published analyses (https://doi.org/10.1016/j.stem.2022.11.004).
Temporal proteomic profiling of iPSC-derived neurons from the KOLF2.1J parental line over a course of 28-day differentiation (n = 6), including 7 time points (day0 [d0], d3, d7, d10, d14, d21, d28) are also available. The preprint describing the workflow used for these data is available here: (https://doi.org/10.1101/2021.11.24.469921).
The associated Github for these files can be found at https://github.com/NIH-CARD/INDI-README/tree/main/INDI-genetics.
Coverage
Spatial:
US
Follow Up:
Unknown
Provenance
Temporal
Accrual Periodicity:
Static
Start Date:
01 January 2026
Time Lag:
Not applicable
Accessibility
Access
Usage
Resource Creators:
Caroline Pantazis, Mike Nalls
Observations
Statistical Population
Population Description
Population Size
Measured Property
Observation Date
Events
8
Count
01 January 2026