Version: 1.0.0 | Published: 24 Sep 2026 | Updated: 0 days ago
Documentation
Description:
This dataset includes Fastq files and DESeq2 results from humaniPSC-Astrocytesand_iMGL.It contains samples carrying the MAPT IVS10+16 mutation and isogenic controls.
MAPT IVS10+16 mutation carrying GIH36C2 and corresponding CRISPR-Cas9 corrected isogenic control line GIH36C2Δ1D01 were used in this study. Human iPSCs were differentiated into microglia using a two-step protocol through intermediate hematopoietic progenitor cells modified from Blurton-Jones et al. RNA was extracted from iMGLs using RNeasy Mini Kit (Qiagen, Cat#: 74104). Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation. Library preparation was performed using Ribo-ZERO kits (Illumina-EpiCentre). RNA was sequenced on an Illumina NovaSeq-6000 using paired-end reads extending 150 bases. RNA-seq reads were then aligned and quantitated to the Ensembl release 101 primary assembly with an Illumina DRAGEN Bio-IT on-premises server running version 3.9.3-8 software.
Preprint now available: Cell autonomous microglia defects in a stem cell model of frontotemporal dementia (medRxiv 2024.05.15.24307444; doi: https://doi.org/10.1101/2024.05.15.24307444)
Coverage
Spatial:
US
Follow Up:
Unknown
Provenance
Temporal
Accrual Periodicity:
Static
Start Date:
01 January 2026
Time Lag:
Not applicable
Accessibility
Access
Access Rights:
See dataset for details. Access data at:
Usage
Resource Creators:
Celeste Karch
Observations
Statistical Population
Population Description
Population Size
Measured Property
Observation Date
Persons
1
Count
01 January 2026