Version: 1.0.0 | Published: 24 Sep 2026 | Updated: 0 days ago
Documentation
Description:
From the same subject, fresh dermal and frozen dural cell lines were plated into a six-well cell culture plate and isolated for RNA-sequencing. Cells were trypsinized, washed with PBS−/−, and total RNA was extracted and purified using the Quick-RNA Microprep Kit (Zymo Research, Irvine, CA, USA, #R1051). Samples were extracted in quadruplicates and RNA was quantified using a NanoDrop. Libraries for RNA-sequencing were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian library preparation protocol (Takara Bio USA, Inc., Mountain View, CA, USA, #634413), analyzed on Agilent TapeStation 4200 (Agilent Technologies, Inc., Santa Clara, CA, USA, #G2991AA), and pooled before paired-end 150-base-pair sequencing on one lane of an Illumina NovaSeq 6000 sequencer (Illumina, Inc., San Diego, CA, USA), which generated 3 million reads per sample. Salmon (v0.13.1) was used to map reads to the human transcriptome (Gencode v28). Tximport (v1.14.0) was used to import the data into R (v3.6.1) and DESeq2 (v1.26.0) was then used to perform differential gene expression analysis.
Coverage
Spatial:
US
Follow Up:
Unknown
Provenance
Temporal
Accrual Periodicity:
Static
Start Date:
01 January 2026
Time Lag:
Not applicable
Accessibility
Access
Access Rights:
-. Access data at:
Usage
Resource Creators:
Andrea R Argouarch, Kristle Garcia, Hani Goodarzi , Aimee Kao
Observations
Statistical Population
Population Description
Population Size
Measured Property
Observation Date
Persons
1
Count
01 January 2026